Intragenic recombination in the CSR1 locus of Arabidopsis

Mol Gen Genet. 1994 Apr;243(2):178-84. doi: 10.1007/BF00280315.

Abstract

Four classes of herbicides are known to inhibit plant acetolactate synthase (ALS). In Arabidopsis, ALS is encoded by a single gene, CSR1. The dominant csr1-1 allele encodes an ALS resistant to chlorsulfuron and triazolopyrimidine sulfonamide while the dominant csr1-2 allele encodes an ALS resistant to imazapyr and pyrimidyl-oxy-benzoate. The molecular distance between the point mutations in csr1-1 and csr1-2 is 1369 bp. Here we used multiherbicide resistance as a stringent selection to measure the intragenic recombination frequency between these two point mutations. We found this frequency to be 0.008 +/- 0.0028. The recombinant multiherbicide-resistant allele, csr1-4, provides an ideal marker for plant genetic transformation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetolactate Synthase / antagonists & inhibitors
  • Acetolactate Synthase / genetics*
  • Alleles
  • Arabidopsis / genetics*
  • Base Sequence
  • Benzoates / pharmacology
  • Codon
  • DNA Mutational Analysis
  • DNA Primers
  • Drug Resistance / genetics*
  • Genes, Plant / genetics*
  • Genetic Markers
  • Herbicides / pharmacology
  • Homozygote
  • Imidazoles / pharmacology
  • Molecular Sequence Data
  • Niacin / analogs & derivatives
  • Niacin / pharmacology
  • Plant Proteins / genetics
  • Point Mutation
  • Recombination, Genetic*
  • Sulfonamides / pharmacology
  • Transformation, Genetic
  • Triazines / pharmacology

Substances

  • Benzoates
  • Codon
  • DNA Primers
  • Genetic Markers
  • Herbicides
  • Imidazoles
  • Plant Proteins
  • Sulfonamides
  • Triazines
  • Niacin
  • imazapyr
  • Acetolactate Synthase
  • chlorsulfuron