Alteration of collagenous protein profile in congestive heart failure secondary to myocardial infarction

Mol Cell Biochem. 1993 Dec 22;129(2):121-31. doi: 10.1007/BF00926360.

Abstract

The rat model of myocardial infarction is characterized by progressive cardiac hypertrophy and failure. Rats with infarcts greater than 30% of the left ventricle exhibited early and moderate stages of heart failure 4 and 8 weeks after the occlusion of the left coronary artery, respectively. As heart failure is usually associated with remodeling of the extracellular matrix, a histological and biochemical study of cardiac collagenous proteins was carried out using failing hearts. Total collagen content in the right ventricle increased at 2, 4, and 8 weeks following occlusion of the left coronary artery whereas such a change in viable left ventricle was seen after 4 and 8 weeks. Total cardiac hydroxyproline concentration was increased in both right and left ventricular samples from the infarcted animals when compared to those of control; this increase was due to elevation of pepsin-insoluble collagen fraction. The myocardial noncollagenous/collagenous protein ratio was decreased in experimental right and left ventricular samples when compared to control samples. These findings suggest that an increase in cross-linking of cardiac collagen as well as disparate synthesis of collagenous and noncollagenous proteins occurs in this model of congestive heart failure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Collagen / metabolism*
  • Heart Failure / etiology
  • Heart Failure / metabolism*
  • Heart Failure / pathology
  • Male
  • Myocardial Infarction / complications
  • Myocardial Infarction / metabolism*
  • Myocardial Infarction / pathology
  • Rats
  • Rats, Sprague-Dawley
  • Solubility

Substances

  • Collagen