Isolation and characterization of the intracellular MHC class II compartment

Nature. 1994 May 12;369(6476):120-6. doi: 10.1038/369120a0.


An intracellular compartment has been isolated to which MHC class II molecules are transported on their way to the plasma membrane. They arrive with an associated invariant chain which is then proteolytically processed while MHC class II molecules acquire antigenic peptide. These loaded class II molecules then leave the compartment devoid of invariant chain and bound for the plasma membrane. This compartment represents a new stage in the endocytic/lysosomal pathway.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Differentiation, B-Lymphocyte*
  • Biological Transport
  • Cell Compartmentation*
  • Cell Fractionation
  • Cell Line
  • Cell Membrane / metabolism
  • Electrophoresis / methods
  • Endocytosis
  • Histocompatibility Antigens Class II / metabolism*
  • Humans
  • Lysosomes
  • Proteins / metabolism
  • Sodium Dodecyl Sulfate
  • Tumor Cells, Cultured


  • Antigens, Differentiation, B-Lymphocyte
  • Histocompatibility Antigens Class II
  • Proteins
  • invariant chain
  • Sodium Dodecyl Sulfate