The Glycoprotein B Genes of Marek's Disease Virus Serotypes 2 and 3: Identification and Expression by Recombinant Fowlpox Viruses

Virology. 1994 May 1;200(2):484-93. doi: 10.1006/viro.1994.1211.

Abstract

The nucleotide sequences of the glycoprotein B (gB) genes of Marek's disease virus (MDV) serotypes 2 and 3 were determined (gB-2 and gB-3, respectively). The genomic locations of these genes coincide with that of the gB gene of serotype 1 MDV (gB-1). Alignment with gB-1 (Ross et al., 1989, J. Gen. Virol. 70, 1789-1804) revealed predicted amino acid identities of 83 and 82% for gB-2 and gB-3, respectively. Excluding the predicted N-terminal signal sequences, 8 of 9 potential N-linked glycosylation sites and all 10 cysteine residues in gB-1 are conserved in both gB-2 and gB-3. In addition, the putative proteolytic cleavage sites for processing of precursors (gp100s) to gp60s and gp49s are conserved among the three gB homologs. Fowlpox virus (FPV) recombinants expressing either the gB-2 or the gB-3 gene were constructed. We detected expression of authentic gB-2 and gB-3 complexes in cells infected with these FPV recombinants. Digestion of immunoprecipitated gB-1 and gB-3 with endoglycosidases revealed that both gp60s are modified by the additions of O-glycans and complex carbohydrates after cleavage of gp100s, while gp100s and gp49s contain only high-mannose carbohydrates. We confirm that the size differences between gB-1 and gB-3 complexes are due to different carbohydrate modifications.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens, Viral / genetics*
  • Antigens, Viral / immunology
  • Antigens, Viral / metabolism
  • Base Sequence
  • Carbohydrate Metabolism
  • Fowlpox virus / genetics
  • Gene Expression
  • Genes, Viral / genetics*
  • Glycoside Hydrolases / metabolism
  • Herpesvirus 2, Gallid / genetics*
  • Herpesvirus 2, Gallid / immunology
  • Molecular Sequence Data
  • Polysaccharides / metabolism
  • Recombinant Proteins / biosynthesis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Serotyping
  • Viral Envelope Proteins / genetics*
  • Viral Envelope Proteins / immunology
  • Viral Envelope Proteins / metabolism

Substances

  • Antigens, Viral
  • Polysaccharides
  • Recombinant Proteins
  • Viral Envelope Proteins
  • glycoprotein B, Marek's disease virus
  • Glycoside Hydrolases

Associated data

  • GENBANK/U01886
  • GENBANK/U01887