Human intestinal VIP receptor: cloning and functional expression of two cDNA encoding proteins with different N-terminal domains

Biochem Biophys Res Commun. 1994 Apr 29;200(2):769-76. doi: 10.1006/bbrc.1994.1517.


We describe here two human VIP receptor cDNA clones isolated from a jejunal epithelial cell cDNA library. The hIVR8 cDNA encodes a human VIP receptor consisting of 460 amino acids and has seven putative transmembrane domains like other G protein-coupled receptors. When expressed in COS-7 cells, hIVR8 conferred specific [125I]VIP binding sites (dissociation constant = 0.6 nM) with displacement patterns characteristic of the human common VIP/PACAP receptor, i.e., VIP = PACAP-27 > PACAP-38 > helodermin > growth hormone-releasing factor = peptide methionineamide > secretin. It also conferred stimulation of cAMP production by VIP (half-maximal stimulation for 0.5 nM peptide). Another clone, hIVR5, encodes a 495 amino acid VIP receptor-related protein exhibiting 100% homology with the functional VIP receptor (hIVR8) over the 428 amino acids at the C-terminus but a completely divergent 67 amino acid N-terminal domain. When expressed in COS-7 cells, this VIP receptor-related protein does not bind 125I-VIP, although it is normally addressed at the plasma membrane as assessed by immunofluorescence studies.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • Cyclic AMP / biosynthesis
  • DNA, Complementary / genetics*
  • Epithelium / metabolism
  • Gene Expression
  • Humans
  • Jejunum / drug effects
  • Jejunum / metabolism*
  • Kinetics
  • Molecular Sequence Data
  • Receptors, Vasoactive Intestinal Peptide / drug effects
  • Receptors, Vasoactive Intestinal Peptide / genetics*
  • Receptors, Vasoactive Intestinal Peptide / metabolism
  • Transfection
  • Vasoactive Intestinal Peptide / metabolism
  • Vasoactive Intestinal Peptide / pharmacology


  • DNA, Complementary
  • Receptors, Vasoactive Intestinal Peptide
  • Vasoactive Intestinal Peptide
  • Cyclic AMP

Associated data

  • GENBANK/X75299
  • GENBANK/X77777