Identification of novel cDNAs encoding human kexin-like protease, PACE4 isoforms

Biochem Biophys Res Commun. 1994 Apr 29;200(2):943-50. doi: 10.1006/bbrc.1994.1541.

Abstract

PACE4 has been identified as a second human subtilisin-like protease by Keifer et al. [DNA and Cell Biology (1991) 10, 757-769]. In this study, we isolated two novel cDNAs coding for PACE4 isoforms (PACE4C and PACE4D) from a human placenta cDNA library. The deduced PACE4C protein sequence (652 amino acids) lacks the cysteine-rich region located at the carboxy terminus of PACE4. The DNA sequence of the 3'-untranslated region (1 kb) of PACE4C is not homologous to the PACE4 sequence. The protein (497 amino acids) encoded by PACE4D cDNA lacks a signal peptide, a propeptide and the cysteine-rich region. These isoform cDNAs were also isolated from rat pituitary cDNA library.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA Primers / genetics
  • DNA, Complementary / genetics*
  • Female
  • Gene Library
  • Humans
  • Isoenzymes / genetics*
  • Molecular Sequence Data
  • Pituitary Gland, Anterior / enzymology
  • Placenta / enzymology
  • Pregnancy
  • Proprotein Convertases
  • Rats
  • Serine Endopeptidases / genetics*

Substances

  • DNA Primers
  • DNA, Complementary
  • Isoenzymes
  • PCSK6 protein, human
  • Pcsk6 protein, rat
  • Proprotein Convertases
  • Serine Endopeptidases

Associated data

  • GENBANK/D28513
  • GENBANK/D28514