Expression of the integrin alpha 4 beta 1 on melanoma cells can inhibit the invasive stage of metastasis formation

Cell. 1994 May 6;77(3):335-47. doi: 10.1016/0092-8674(94)90149-x.

Abstract

Among a series of adhesion molecules, expression of integrin alpha 4 beta 1 showed a unique inverse correlation with the invasive potential of B16 melanoma cell lines. When an alpha 4 cDNA was introduced into an alpha 4-beta 1+ highly invasive melanoma line, alpha 4 beta 1 heterodimers were expressed on the surface. Matrigel invasion by the alpha 4+ beta 1+ cells was reduced. Pulmonary metastasis was also suppressed when the transfectants were placed subcutaneously, but not when injected intravenously. Expression of alpha 4 beta 1 promoted homotypic intercellular adhesion. The homotypic adhesion was abrogated, and the alpha 4+ beta 1+ (less invasive cell lines) increased matrigel invasion following the anti-alpha 4 MAb treatment. These results suggest that integrin alpha 4 beta 1 could play a role in controlling melanoma cell metastasis at the invasive stage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Cell Adhesion / physiology
  • Cell Adhesion Molecules / analysis
  • Collagen
  • DNA, Complementary
  • Drug Combinations
  • Genetic Vectors
  • Integrin alpha4beta1
  • Integrins / analysis
  • Integrins / biosynthesis*
  • Integrins / genetics
  • Laminin
  • Lung Neoplasms / secondary
  • Male
  • Melanoma, Experimental / metabolism*
  • Melanoma, Experimental / pathology
  • Melanoma, Experimental / secondary
  • Mice
  • Mice, Inbred C57BL
  • Neoplasm Invasiveness*
  • Proteoglycans
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • Receptors, Very Late Antigen / analysis
  • Receptors, Very Late Antigen / biosynthesis*
  • Receptors, Very Late Antigen / genetics
  • Retroviridae / genetics
  • Tumor Cells, Cultured

Substances

  • Antibodies, Monoclonal
  • Cell Adhesion Molecules
  • DNA, Complementary
  • Drug Combinations
  • Integrin alpha4beta1
  • Integrins
  • Laminin
  • Proteoglycans
  • RNA, Messenger
  • Receptors, Very Late Antigen
  • matrigel
  • Collagen