The human interferon alpha/beta receptor: characterization and molecular cloning

Cell. 1994 May 6;77(3):391-400. doi: 10.1016/0092-8674(94)90154-6.


We describe a universal ligand-binding receptor for human interferons alpha and interferon beta (type I IFNs). A soluble 40 kDa IFN-alpha/beta receptor (p40) that blocks the activity of type I IFNs was purified from urine and sequenced. Antibodies raised against p40 completely block the activity of several type I IFNs and immuno-precipitate both a cellular 102 kDa IFN-alpha/beta receptor and its cross-linked complexes with IFN-alpha 2. The receptor is a disulfide-linked dimer, consisting of 51 kDa subunits. We isolated and expressed a 1.5 kb cDNA, coding for the IFN-alpha/beta receptor. Its 331 amino acid sequence includes a leader and a transmembrane region, while its ectodomain corresponds to p40. IFN-alpha/beta receptor is physically associated with the cytoplasmic Tyr kinase JAK1, hence, in addition to ligand binding, it is directly involved in signal transduction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Amino Acids / analysis
  • Animals
  • Antibodies
  • Base Sequence
  • Cell Membrane / chemistry
  • Cloning, Molecular
  • DNA, Complementary / analysis
  • Humans
  • Interferon Type I / metabolism*
  • Membrane Proteins
  • Mice
  • Molecular Sequence Data
  • Neutralization Tests
  • Protein-Tyrosine Kinases / analysis
  • Rabbits
  • Radioligand Assay
  • Receptor, Interferon alpha-beta
  • Receptors, Interferon / analysis
  • Receptors, Interferon / chemistry
  • Receptors, Interferon / genetics*
  • Receptors, Interferon / immunology
  • Receptors, Interferon / isolation & purification
  • Receptors, Interferon / metabolism*
  • Sequence Analysis
  • Sequence Analysis, DNA


  • Amino Acids
  • Antibodies
  • DNA, Complementary
  • Interferon Type I
  • Membrane Proteins
  • Receptors, Interferon
  • Receptor, Interferon alpha-beta
  • Protein-Tyrosine Kinases

Associated data

  • GENBANK/X77722