Cytokine, complement, and endotoxin profiles associated with the development of the adult respiratory distress syndrome after severe injury

Crit Care Med. 1994 May;22(5):768-76. doi: 10.1097/00003246-199405000-00010.

Abstract

Objective: The adult respiratory distress syndrome (ARDS) is a frequent complication after severe accidental trauma. This study examines the hypothesis that increased systemic concentrations of proinflammatory cytokines, endotoxin, or complement fragments may predict the development of ARDS.

Design: Prospective, observational study.

Setting: Two Level I university trauma centers.

Patients: Fifteen severely injured patients (Injury Severity Score of > or = 25).

Interventions: Standard emergency department, operating room, and intensive care unit management.

Measurements and main results: Plasma samples were obtained at 4-hr intervals from the time of injury and were assayed for concentrations of endotoxin, tumor necrosis factor-alpha, interleukin (IL)-1 beta, IL-6, IL-8, and complement fragments C3a and C4a. Hemodynamic and oxygen metabolism variables also were measured at 4-hr intervals after injury. Seven patients developed ARDS and eight patients did not. The PaO2/FIO2 ratio was significantly decreased in the patients with ARDS compared with non-ARDS patients as early as 4 hrs postinjury, and remained significantly decreased throughout the initial 24 hrs after severe accidental injury. Plasma IL-8, IL-6, C3a, and C4a concentrations were markedly increased starting in the immediate postinjury period in both ARDS and non-ARDS patients, but no significant differences were found between the two groups until 16 hrs after injury when plasma IL-8, C3a, and C4a concentrations became significantly higher in the ARDS group. Neither the ARDS nor non-ARDS patients showed the presence of circulating IL-1 beta, TNF-alpha, or endotoxin at any postinjury time point.

Conclusions: These results demonstrate that measurements of plasma concentrations of proinflammatory cytokines, endotoxin, or complement fragments are not helpful in predicting the development of ARDS after severe accidental injury.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Blood Gas Analysis
  • Complement C3a / analysis*
  • Complement C4a / analysis*
  • Craniocerebral Trauma / classification
  • Craniocerebral Trauma / complications*
  • Endotoxins / blood*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Hemodynamics*
  • Humans
  • Injury Severity Score
  • Interleukin-1 / blood*
  • Interleukin-1beta
  • Interleukin-6 / blood*
  • Interleukin-8 / blood*
  • Male
  • Middle Aged
  • Oxygen Consumption
  • Peptide Fragments / blood*
  • Predictive Value of Tests
  • Prognosis
  • Prospective Studies
  • Radioimmunoassay
  • Respiratory Distress Syndrome / blood*
  • Respiratory Distress Syndrome / epidemiology*
  • Respiratory Distress Syndrome / etiology
  • Respiratory Distress Syndrome / physiopathology
  • Risk Factors
  • Survival Rate
  • Time Factors
  • Tumor Necrosis Factor-alpha / analysis*

Substances

  • Endotoxins
  • Interleukin-1
  • Interleukin-1beta
  • Interleukin-6
  • Interleukin-8
  • Peptide Fragments
  • Tumor Necrosis Factor-alpha
  • interleukin-1beta (163-171)
  • Complement C3a
  • Complement C4a