Comparative analysis of a biofilm-forming Staphylococcus epidermidis strain and its adhesion-positive, accumulation-negative mutant M7

FEMS Microbiol Lett. 1994 Mar 15;117(1):71-8. doi: 10.1111/j.1574-6968.1994.tb06744.x.


We have isolated a stable slime-negative mutant, M7, from the wild-type Staphylococcus epidermidis RP62A by mitomycin mutagenesis. Besides its inability to produce slime in the test tube this mutant differed also in two other properties from its parent strain: it lacked the ability to accumulate on a surface, and it did not produce a 115 kDa and a 18 kDa extracellular protein. In all other tested properties such as initial adherence, growth rate, cell-wall composition, surface characteristics, DNA restriction profile, the presence of a 29 kb antibiotic resistance plasmid, and antimicrobial susceptibility profile, M7 was indistinguishable from its wild-type. The mutant is an important basis for further study of the pathogenesis of polymer-associated S. epidermidis infections.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Adhesion / genetics
  • Bacterial Adhesion / physiology
  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / genetics
  • Biopolymers / biosynthesis*
  • Biopolymers / genetics*
  • Humans
  • Mutation
  • Staphylococcal Infections / etiology
  • Staphylococcus epidermidis / genetics*
  • Staphylococcus epidermidis / metabolism*
  • Staphylococcus epidermidis / pathogenicity


  • Bacterial Proteins
  • Biopolymers