Nucleotide sequence of the structural gene for the penicillin-binding protein 2 of Staphylococcus aureus and the presence of a homologous gene in other staphylococci

FEMS Microbiol Lett. 1994 Apr 1;117(2):131-6. doi: 10.1111/j.1574-6968.1994.tb06754.x.

Abstract

The structural gene for penicillin-binding protein 2 (PBP2) of Staphylococcus aureus was cloned and sequenced. The nucleotide sequence of the 2,458-bp chromosomal fragment was determined, and the 2,148-bp coding region for PBP2 was identified. Determination of ten N-terminal amino acids of the PBP2 protein indicated that N-terminal methionine had been removed from the primary translational product. Thus, PBP2 is comprised of 715 amino acids with a molecular mass of 79,147. Nucleotide sequences having some homology with the PBP2 gene and proteins cross-reactive with anti-PBP2 antibody were detected in some other species of staphylococci by polymerase chain reaction and Western blot analysis, respectively.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins*
  • Base Sequence
  • Blotting, Western
  • Carrier Proteins*
  • Cloning, Molecular
  • Cross Reactions
  • Genes, Bacterial*
  • Hexosyltransferases / genetics*
  • Hexosyltransferases / immunology
  • Molecular Sequence Data
  • Multienzyme Complexes / genetics*
  • Multienzyme Complexes / immunology
  • Muramoylpentapeptide Carboxypeptidase*
  • Penicillin-Binding Proteins
  • Peptidyl Transferases / genetics*
  • Peptidyl Transferases / immunology
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Staphylococcus / genetics*
  • Staphylococcus aureus / genetics*

Substances

  • Bacterial Proteins
  • Carrier Proteins
  • Multienzyme Complexes
  • Penicillin-Binding Proteins
  • Peptidyl Transferases
  • Hexosyltransferases
  • Muramoylpentapeptide Carboxypeptidase

Associated data

  • GENBANK/X62288