PAD1 Encodes Phenylacrylic Acid Decarboxylase Which Confers Resistance to Cinnamic Acid in Saccharomyces Cerevisiae

Gene. 1994 May 3;142(1):107-12. doi: 10.1016/0378-1119(94)90363-8.

Abstract

The yeast enzyme phenylacrylic acid decarboxylase (PAD) confers resistance to phenylacrylic acids. Cinnamic acid (CA)-sensitive mutants lacking PAD activity were isolated and the PAD1 gene was cloned by phenotypic complementation. The nucleotide sequence of the smallest complementing fragment was determined. The predicted 242-amino-acid PAD polypeptide is 48.6% identical to the product of dedF of Escherichia coli. PAD activity and CA resistance, but not steady-state PAD1 mRNA levels, are influenced by mitochondrial genotype. PAD1 is a single-copy gene in the yeast genome and not essential for viability. The PAD1 locus was physically mapped to a position approx. 140 kb from the left end of chromosome IV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carboxy-Lyases / genetics*
  • Cinnamates / pharmacology*
  • DNA, Fungal
  • Drug Resistance, Microbial / genetics
  • Fungal Proteins / genetics*
  • Molecular Sequence Data
  • Restriction Mapping
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*

Substances

  • Cinnamates
  • DNA, Fungal
  • Fungal Proteins
  • Carboxy-Lyases
  • phenylacrylic acid decarboxylase
  • cinnamic acid

Associated data

  • GENBANK/L09263