Abstract
Nuclear Factor I (NFI) proteins are DNA binding proteins functioning as transcription and replication factors. As part of a study of the diversity of the Nuclear Factor I protein family, we isolated and sequenced seven NFI cDNA clones from a chicken promacrophage library. Five of these clones are derived from the NFI-A gene, the other two from the NFI-C gene. Comparison of the deduced chicken NFI amino acid sequences with mammalian NFI sequences reveals that there are corresponding vertebrate isoforms, indicating a strong conservation of NFI structure during evolution. This finding leads to the prediction that more mammalian isoforms will be discovered corresponding to the chicken NFI variants reported here.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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CCAAT-Enhancer-Binding Proteins*
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Chickens
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Cloning, Molecular
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DNA, Complementary / analysis
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DNA-Binding Proteins / genetics*
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Mammals
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Molecular Sequence Data
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NFI Transcription Factors
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Nuclear Proteins
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RNA Splicing / genetics*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription Factors / genetics*
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Y-Box-Binding Protein 1
Substances
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CCAAT-Enhancer-Binding Proteins
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DNA, Complementary
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DNA-Binding Proteins
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NFI Transcription Factors
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Nuclear Proteins
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Transcription Factors
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Y-Box-Binding Protein 1
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YBX1 protein, human
Associated data
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GENBANK/X51486
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GENBANK/X68152
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GENBANK/X68153
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GENBANK/X68154
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GENBANK/X68155
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GENBANK/X68156
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GENBANK/X68157
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GENBANK/X68158