Association of a host DNA structure with retroviral integration sites in chromosomal DNA

Virology. 1994 Jun;201(2):408-12. doi: 10.1006/viro.1994.1310.

Abstract

Integration of retroviral genomes is a site-specific process with respect to the virus but not the host genome. Numerous chromosomal sites and various sequences can be used as targets. Nevertheless, preferential regions and integration patterns have been observed. Using a functional assay, we investigated if host structural DNA elements could be associated with retroviral integration sites. The results were that 9 of 10 distinct retroviral integration events occurred in close proximity of structural elements behaving like intrinsically bent DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromosomes
  • Chromosomes, Human
  • DNA / chemistry*
  • DNA / genetics
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Nucleic Acid Conformation*
  • Restriction Mapping
  • Retroviridae / genetics*
  • Retroviridae / physiology
  • Virus Integration*

Substances

  • DNA