Tissue-specific constitutive and inducible expression of rat phenol UDP-glucuronosyltransferase

Biochem Pharmacol. 1994 Apr 20;47(8):1445-8. doi: 10.1016/0006-2952(94)90345-x.


To investigate constitutive and inducible expression of rat phenol UDP-glucuronosyltransferase (UGT1A1) in liver and extrahepatic tissues, a selective cDNA probe for its unique exon 1 was utilized. 6-Hydroxychrysene was used as a functional probe of UGT1A1 activity. Constitutive expression of UGT1A1 was low in liver, but high in kidney, testis, epididymis and ovary. After treatment with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; 10 micrograms/kg for 7 days) the UGT1A1 mRNA level was markedly increased in liver (ca. 10-fold), and only moderately enhanced (up to 2-fold) in extrahepatic tissues where constitutive enzyme expression was high. UGT activity toward 6-hydroxychrysene was strongly inducible in liver (ca. 9-fold) and only moderately inducible in extrahepatic tissues (up to 2-fold). The results suggest complex tissue-specific regulation of UGT1A1 including positive and negative transcriptional factors and marked inducibility by TCDD in liver.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chrysenes / metabolism
  • Female
  • Gene Expression / drug effects
  • Glucuronosyltransferase / genetics
  • Glucuronosyltransferase / metabolism*
  • Liver / drug effects
  • Liver / enzymology*
  • Male
  • Polychlorinated Dibenzodioxins / pharmacology*
  • RNA, Messenger / analysis
  • Rats
  • Rats, Wistar


  • Chrysenes
  • Polychlorinated Dibenzodioxins
  • RNA, Messenger
  • 6-hydroxychrysene
  • Glucuronosyltransferase