Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells

Biochem Pharmacol. 1994 Apr 29;47(9):1601-6. doi: 10.1016/0006-2952(94)90538-x.

Abstract

MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer cell line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance was due to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Drug Resistance / genetics*
  • Gene Expression
  • Humans
  • Mitoxantrone / pharmacology*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis*
  • Tumor Cells, Cultured

Substances

  • RNA, Messenger
  • Mitoxantrone