The Aqueous Solution Structure of the Tetrasaccharide-Ribitol Repeat-Unit From the Lipoteichoic Acid of Streptococcus Pneumoniae Strain R6 Determined Using a Combination of NMR Spectroscopy and Computer Calculations

Carbohydr Res. 1994 Apr 1;256(2):189-222. doi: 10.1016/0008-6215(94)84209-4.

Abstract

High-resolution 1D- and 2D-correlation 1H NMR and 13C NMR, at 500 and 125 MHz, respectively, permitted assignment of the majority of the resonances in the per-N-acetylated, phosphorylated tetrasaccharide-ribitol repeat-unit, and in the complete polymer (n = 5 - 7) containing between five and seven repeating units attached to the deacylated lipid anchor, for the lipoteichoic acid from Streptococcus pneumoniae strain R6; the 31P resonances were also assigned. Comparison of the 31P spectra obtained for the per-N-acetylated oligosaccharide and for the oligosaccharide having the AATG 4-NH2 group still free, indicate a conformational difference brought about by interaction between the amino group and the neighboring phosphate group.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Computer Simulation
  • Forssman Antigen / chemistry*
  • Lipopolysaccharides / chemistry*
  • Magnetic Resonance Spectroscopy
  • Micelles
  • Models, Chemical
  • Models, Molecular
  • Molecular Sequence Data
  • Oligosaccharides / chemistry*
  • Phosphorylcholine / chemistry
  • Ribitol / chemistry
  • Streptococcus pneumoniae / chemistry*
  • Teichoic Acids / chemistry*

Substances

  • Lipopolysaccharides
  • Micelles
  • Oligosaccharides
  • Teichoic Acids
  • Phosphorylcholine
  • Ribitol
  • lipoteichoic acid
  • Forssman Antigen