The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene

Cell. 1994 May 20;77(4):579-86. doi: 10.1016/0092-8674(94)90219-4.


The S. cerevisiae VPS10 (vacuolar protein sorting) gene encodes a type I transmembrane protein of 1577 amino acids required for the sorting of the soluble vacuolar protein carboxypeptidase Y (CPY). Mutations in VPS10 result in the selective missorting and secretion of CPY; all other vacuolar proteins tested are delivered to the vacuole in vps10 mutants. Chemical cross-linking studies demonstrate that Vps10p and the Golgi-modified precursor form of CPY directly interact. A single amino acid change in the CPY vacuolar sorting signal prevents this interaction. Vps10p also interacts with a hybrid protein containing the CPY sorting signal fused to the normally secreted enzyme invertase. Subcellular fractionation indicates that the majority of Vps10p is localized to a late Golgi compartment where vacuolar proteins are sorted. We propose that VPS10 encodes a CPY sorting receptor that executes multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Carboxypeptidases / analysis
  • Carboxypeptidases / metabolism*
  • Cathepsin A
  • Cloning, Molecular
  • Fungal Proteins / analysis
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Genes, Fungal / genetics*
  • Genetic Complementation Test
  • Glycosylation
  • Golgi Apparatus / chemistry
  • Membrane Proteins / analysis
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Models, Biological
  • Molecular Sequence Data
  • Mutation / physiology
  • Receptors, Cell Surface / analysis
  • Receptors, Cell Surface / chemistry
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / metabolism
  • Receptors, Cytoplasmic and Nuclear / analysis
  • Receptors, Cytoplasmic and Nuclear / genetics*
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Spheroplasts / chemistry
  • Vacuoles / chemistry
  • Vacuoles / metabolism*
  • Vesicular Transport Proteins*


  • Fungal Proteins
  • Membrane Proteins
  • PEP1 protein, S cerevisiae
  • Receptors, Cell Surface
  • Receptors, Cytoplasmic and Nuclear
  • Saccharomyces cerevisiae Proteins
  • Vesicular Transport Proteins
  • Carboxypeptidases
  • Cathepsin A
  • PRC1 protein, S cerevisiae
  • serine carboxypeptidase

Associated data

  • GENBANK/U07621