A new genomic species in Borrelia burgdorferi sensu lato isolated from Japanese ticks

Res Microbiol. Jul-Aug 1993;144(6):467-73. doi: 10.1016/0923-2508(93)90054-6.


Five Borrelia strains (Ika2, HO14, Cow611C, 0612 and F63B) isolated from Ixodes ovatus ticks in Japan were analysed by DNA-DNA hybridization experiments, ribotyping, pulsed-field gel electrophoresis and protein electrophoresis. DNA relatedness set these strains in a new genomic species within the Borrelia burgdorferi complex; this species appears to be restricted to Japan and could be non-pathogenic for humans. The ribotype and pulsotype of strain Ika2 were atypical of the new genomic species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / analysis
  • Borrelia burgdorferi Group / genetics
  • Borrelia burgdorferi Group / isolation & purification*
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • Electrophoresis, Gel, Pulsed-Field
  • Electrophoresis, Polyacrylamide Gel
  • In Vitro Techniques
  • Japan
  • Lyme Disease / microbiology*
  • Nucleic Acid Hybridization / methods*
  • RNA, Ribosomal / analysis
  • RNA, Ribosomal / genetics
  • Restriction Mapping
  • Ticks / microbiology*


  • Bacterial Proteins
  • DNA, Bacterial
  • RNA, Ribosomal