Sphinganine 1-phosphate metabolism in cultured skin fibroblasts: evidence for the existence of a sphingosine phosphatase

Biochem J. 1994 May 1;299 ( Pt 3)(Pt 3):597-601. doi: 10.1042/bj2990597.


On addition of [4,5-3H]sphinganine 1-phosphate to human fibroblast monolayers, the label was efficiently removed from the culture medium. In contrast with the reported stability of phosphorylated sphingenine in 3T3 cells [Desai, Zhang, Olivera, Mattie and Spiegel (1992). J. Biol. Chem. 267, 23122-23128] and B16 melanoma cells [Sadahira, Ruan, Hakomuri and Igarashi (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 9686-9690], sphinganine 1-phosphate appeared to be subjected to a fast and extensive metabolism in fibroblasts, the major pathways being cleavage and dephosphorylation. The first of these pathways, catalysed by sphingosine-phosphate lyase, resulted in the formation of labelled palmitaldehyde, which was recovered, mainly after oxidation, in glycerophospholipids in an ester bond. A smaller part of the palmitaldehyde was reduced and incorporated in alk(en)ylphospholipids. Dephosphorylation of spinganine 1-phosphate, a hitherto overlooked pathway catalysed by an unknown phosphatase(s), gave rise to sphinganine, which was converted by N-acylation into ceramide and then incorporated in spingomyelin and glycosphingolipids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenoleukodystrophy / enzymology
  • Adrenoleukodystrophy / metabolism
  • Cells, Cultured
  • Child
  • Fibroblasts / enzymology
  • Humans
  • Hydrolysis
  • Oxidation-Reduction
  • Phosphoric Monoester Hydrolases / metabolism*
  • Phosphorylation
  • Saccharomyces cerevisiae Proteins*
  • Sphingosine / analogs & derivatives*
  • Sphingosine / metabolism
  • Zellweger Syndrome / enzymology
  • Zellweger Syndrome / metabolism


  • Saccharomyces cerevisiae Proteins
  • dihydrosphingosine 1-phosphate
  • LCB3 protein, S cerevisiae
  • YSR3 protein, S cerevisiae
  • Phosphoric Monoester Hydrolases
  • Sphingosine
  • safingol