Abstract
Seven GATC sites that are nonmethylated in logarithmic growth phase cells using glycerol as a carbon source were isolated from the Escherichia coli chromosome. Three of these GATC sites are located upstream of the operons gut, mtl, and ppiA, whereas DNA sequences adjacent to three other nonmethylated GATC sites are not homologous to previously identified genes. The seventh nonmethylated GATC site is located downstream of uspA. The protection of this site from DNA methylation requires leucine-responsive regulatory protein and is leucine responsive. The carbon source and the growth phase influenced the protection of the GATC site 5' of the ppiA gene. The other five sites were protected under all the environmental conditions examined.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Proteins / metabolism
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Base Sequence
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Chromosome Mapping
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Cloning, Molecular
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DNA, Bacterial / genetics*
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DNA, Bacterial / metabolism*
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DNA-Binding Proteins / metabolism
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Escherichia coli / drug effects
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Escherichia coli / genetics*
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Escherichia coli / metabolism
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Escherichia coli Proteins
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Leucine / pharmacology
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Leucine-Responsive Regulatory Protein
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Methylation
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Methyltransferases / metabolism*
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Molecular Sequence Data
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Operon / genetics
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Signal Transduction
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Site-Specific DNA-Methyltransferase (Adenine-Specific)*
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Transcription Factors*
Substances
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Bacterial Proteins
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DNA, Bacterial
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DNA-Binding Proteins
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Escherichia coli Proteins
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Lrp protein, E coli
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Transcription Factors
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Leucine-Responsive Regulatory Protein
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Methyltransferases
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Dam methyltransferase
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Site-Specific DNA-Methyltransferase (Adenine-Specific)
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dam protein, E coli
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Leucine
Associated data
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GENBANK/L31368
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GENBANK/L31369
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GENBANK/L31370
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GENBANK/L31371