The genus Longibucca Chitwood, 1933 (Nematoda: Rhabditoidea) is reviewed based on examination of museum and adult specimens collected from 4 species of bats (Myotis lucifugus, Myotis ciliolabrum, Eptesicus fuscus, and Lasionycteris noctivagans) in Alberta, Canada. Two species are considered valid, namely Longibucca vivipara Chitwood, 1933, and Longibucca lasiura McIntosh and Chitwood, 1934. Longibucca eptesica Elsea, 1953 is considered a synonym of L. lasiura. New hosts of Longibucca lasiura include Pipistrellus subflavus, Lasionycteris noctivagans, and Myotis ciliolabrum. New geographic ranges of Longibucca lasiura are Virginia, U.S.A., Canada, and western North America.