Blockade of D-1 dopamine receptors in the medial prefrontal cortex produces delayed effects on pre- and postsynaptic indices of dopamine function in the nucleus accumbens

Synapse. 1994 Feb;16(2):104-12. doi: 10.1002/syn.890160204.


The present experiments assessed the acute and delayed effects of D-1 dopamine (DA) receptor blockade in the medial prefrontal cortex (mPFC) on pre- and postsynaptic indices of DA neurotransmission in the nucleus accumbens (N.Acc). Different groups of rats received intra-mPFC injections of saline (control animals) or the D-1 DA receptor antagonist SCH-23390 (0.25 microgram/side). Acutely, intra-mPFC injections of this antagonist did not affect spontaneous locomotion but significantly increased the locomotion induced by intra-N.Acc. amphetamine (1.5 micrograms/side), in agreement with our earlier findings [Vezina et al. (1991) Eur. J. Neurosci., 3:1001-1007]. When tested two days post-injection, however, mPFC-SCH-23390 preexposed animals showed lower levels of locomotor activity than Control animals in response to intra-N.Acc. injections of amphetamine. This effect was not observed in other animals preexposed two days earlier to mPFC injections of amphetamine (2.5 micrograms/side) or the D-2 DA receptor antagonist sulpiride (1.0 microgram/side). Animals preexposed two days earlier to mPFC SCH-23390 also showed higher levels of locomotor activity (+98%) when tested with intra-N.Acc. injections of the D-1 DA receptor agonist SKF-38393 (1.0 microgram/side) and a 36% increase in maximal DA-sensitive adenylate cyclase activity in comparison to Control animals. These effects were no longer observed in animals tested seven days following the mPFC SCH-23390 injections. These results demonstrate delayed actions resulting from cortical D-1 DA receptor blockade.(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylyl Cyclases / metabolism
  • Amphetamine / pharmacology
  • Animals
  • Benzazepines / pharmacology
  • Diffusion
  • Dopamine / pharmacology
  • Dopamine / physiology*
  • Dopamine Antagonists*
  • Injections
  • Male
  • Motor Activity / drug effects
  • Nucleus Accumbens / physiology*
  • Prefrontal Cortex / metabolism*
  • Presynaptic Terminals / physiology*
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Dopamine*
  • Synapses / physiology*
  • Time Factors


  • Benzazepines
  • Dopamine Antagonists
  • Receptors, Dopamine
  • dopamine-I receptor
  • Amphetamine
  • Adenylyl Cyclases
  • Dopamine