Cloning and structure of the human corticotrophin releasing factor-binding protein gene (CRHBP)

Genomics. 1993 Apr;16(1):63-8. doi: 10.1006/geno.1993.1141.


The human CRF-binding protein gene has been cloned and mapped to the distal region of chromosome 13 and loci 5q in the mouse and human genomes, respectively. The gene consists of 7 exons and 6 introns. The mature protein has 10 cysteines and 5 tandem disulfide bridges 4 of which are contained within exons 3, 5, 6, and 7. One bridge is shared by exons 3 and 4. The signal peptide and the first 3 amino acids of the mature protein were coded for by an extreme 5' exon. Primer extension analyses revealed the transcriptional initiation site to be located 32 bp downstream from a consensus TATA box. The promoter sequence contained a number of putative promoter elements including an AP-1 site, three ER-half sites, the immunoglobulin enhancer elements NF-kappa B and INF-1, and the liver-specific enhancers LFA1 and LFB1.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Carrier Proteins / genetics*
  • Chromosomes, Human, Pair 5
  • Cloning, Molecular
  • Conserved Sequence
  • Corticotropin-Releasing Hormone / metabolism*
  • DNA / genetics
  • Exons
  • Humans
  • Introns
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Restriction Mapping


  • Carrier Proteins
  • corticotropin releasing factor-binding protein
  • DNA
  • Corticotropin-Releasing Hormone