Colocalization of octopamine and FMRFamide related peptide in identified heart projecting (DUM) neurones in the locust revealed by immunocytochemistry

Brain Res. 1994 Feb 28;638(1-2):117-25. doi: 10.1016/0006-8993(94)90640-8.

Abstract

Immunocytochemical techniques are employed to reveal colocalization of octopamine with FMRFamide related peptide in the locust ventral nervous system. In each unfused pregenital abdominal ganglia (A4-A6) there are 3 octopamine-like immunoreactive neurones. By combining intracellular Lucifer yellow staining with subsequent immunocytochemistry these are individually identified as the efferent dorsal unpaired median (DUM) neurones DUM-1 and DUM-2, which innervate abdominal tergal and respectively sternal skeletal muscles, and DUM heart-1, an FMRFamide-like immunoreactive neurone which projects to the heart and associated alary muscles. Colocalization of octopamine- and FMRFamide-like immunoreactivity in DUM heart-1 is verified by alternate staining of consecutive sections. With respect to locust ventral ganglia, this investigation shows that colocalization of octopamine with an FMRFamide related peptide is restricted to a single DUM cell occurring in each abdominal ganglion 2-7, which most likely corresponds to segmental homologues of DUM heart-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abdomen / innervation
  • Animals
  • Fluorescent Dyes
  • Ganglia, Invertebrate / cytology*
  • Grasshoppers*
  • Heart / innervation
  • Immunohistochemistry / methods
  • Invertebrate Hormones / analysis
  • Isoquinolines
  • Muscles / innervation
  • Neurons / cytology*
  • Neuropeptides / analysis*
  • Octopamine / analysis*
  • Oligopeptides / analysis*

Substances

  • Fluorescent Dyes
  • Invertebrate Hormones
  • Isoquinolines
  • Neuropeptides
  • Oligopeptides
  • Octopamine
  • lucifer yellow
  • phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide