Stimulation of myocyte insulin-responsive glucose transporters by the inhibition of fatty acid oxidation

Diabetes Res. 1993;22(1):11-9.


The effects of the fatty acid inhibitor 4-bromocrotonic acid (4-BCA) on glucose utilization was studied in isolated rat myocytes. In contrast to its potent inhibition of [1-14C]palmitate oxidation, 4-BCA strongly stimulated the oxidation of [1-14C]glucose and [2-14C]-pyruvate in a concentration-dependent manner. At a concentration of 300 microM, 4-BCA increased glucose oxidation threefold and that of pyruvate oxidation twofold. The rate of transport of [U-14C]-2-deoxyglucose was significantly stimulated by 4-BCA. The transport of 2-deoxyglucose was increased sevenfold with 200 microM 4-BCA, whereas insulin (10 microU)/ml enhanced 2-deoxyglucose transport twofold. The addition of insulin to myocytes preincubated with 4-BCA did not further increase glucose transport. Cytochalasin B and anti-GLUT 4 antibody decreased the 4-BCA-induced stimulation of glucose transport. These results suggest that the stimulation of 2-deoxy-glucose transport by 4-BCA occurs through an increase in the activity of insulin-responsive glucose transporters, GLUT 4, in the sarcolemmal membrane.

MeSH terms

  • Animals
  • Biological Transport / drug effects
  • Cells, Cultured
  • Crotonates / pharmacology*
  • Deoxyglucose / metabolism
  • Glucose / metabolism*
  • Glucose Transporter Type 4
  • Heart / drug effects
  • Insulin / pharmacology*
  • Kinetics
  • Male
  • Monosaccharide Transport Proteins / metabolism*
  • Muscle Proteins*
  • Myocardium / metabolism*
  • Oxidation-Reduction
  • Palmitic Acid
  • Palmitic Acids / antagonists & inhibitors
  • Palmitic Acids / metabolism*
  • Pyruvates / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Sarcolemma / drug effects
  • Sarcolemma / metabolism*


  • Crotonates
  • Glucose Transporter Type 4
  • Insulin
  • Monosaccharide Transport Proteins
  • Muscle Proteins
  • Palmitic Acids
  • Pyruvates
  • Slc2a4 protein, rat
  • 4-bromocrotonic acid
  • Palmitic Acid
  • Deoxyglucose
  • Glucose