Pituitary adenylate cyclase-activating polypeptide: effects on pancreatic-adrenal hormone secretion and glucose-lipid metabolism in normal conscious dogs

Metabolism. 1994 Jun;43(6):739-44. doi: 10.1016/0026-0495(94)90124-4.


The effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on plasma insulin, glucagon, catecholamine, cortisol, glucose, triglyceride (TG), free fatty acid (FFA), cholesterol, and cyclic adenosine monophosphate (cAMP) concentrations were examined in unanesthetized normal dogs. A bolus injection of 6 pmol/kg PACAP27 elicited a transient increase in plasma insulin, epinephrine, and norepinephrine concentrations, with a peak value at 2 minutes after injection. Injections of 60 and 600 pmol/kg caused greater increases in these hormone concentrations in a dose-dependent manner. The plasma cortisol concentration was not changed by a bolus injection of 6 pmol/kg PACAP27, and was gradually increased by injections of 60 and 600 pmol/kg. Significant increases were observed from 10 and 5 minutes after the injection of 60 and 600 pmol/kg, respectively. The plasma glucagon concentration was not changed by either 6, 60, or 600 pmol/kg. The plasma glucose concentration decreased with 60 pmol/kg PACAP27 and increased with 600 pmol/kg. The plasma FFA concentration was increased gradually, with a peak value at 10 minutes after the injection, in a dose-dependent manner. The plasma TG concentration was slightly increased with 600 pmol/kg with a peak value at 10 minutes, although plasma cholesterol did not change. The plasma cAMP concentration increased significantly with 600 pmol/kg PACAP27, but not with 6 or 60 pmol/kg. These effects of PACAP27 were observed with a bolus injection of PACAP38 of an equal potency. Infusion of graded doses of PACAP27 (1, 3, and 10 pmol/kg/min every 20 minutes) caused a gradual increase in plasma cortisol, catecholamine, FFA, and cAMP concentrations.(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Glucose / analysis
  • Cholesterol / blood
  • Consciousness / physiology
  • Cyclic AMP / blood
  • Dogs
  • Epinephrine / blood
  • Fatty Acids, Nonesterified / blood
  • Glucagon / blood
  • Glucose / metabolism*
  • Hydrocortisone / blood
  • Infusions, Intravenous
  • Injections, Intravenous
  • Insulin / blood
  • Lipid Metabolism*
  • Neuropeptides / administration & dosage
  • Neuropeptides / pharmacology*
  • Norepinephrine / blood
  • Pituitary Adenylate Cyclase-Activating Polypeptide
  • Triglycerides / blood


  • Blood Glucose
  • Fatty Acids, Nonesterified
  • Insulin
  • Neuropeptides
  • Pituitary Adenylate Cyclase-Activating Polypeptide
  • Triglycerides
  • Glucagon
  • Cholesterol
  • Cyclic AMP
  • Glucose
  • Hydrocortisone
  • Norepinephrine
  • Epinephrine