A novel role for the nitric oxide-cGMP signaling pathway: the control of epithelial function in Drosophila

Am J Physiol. 1994 May;266(5 Pt 2):R1716-9. doi: 10.1152/ajpregu.1994.266.5.R1716.

Abstract

The nitric oxide (NO) signaling pathway plays major roles in the vertebrate vascular, nervous, and immune systems. Here we present evidence that all the elements in the NO pathway are present in, and act to control epithelial fluid secretion by, the Malpighian tubules of an insect, Drosophila melanogaster. This finding will allow both a physiological and a molecular genetic dissection of the NO pathway in the same tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3',5'-Cyclic-GMP Phosphodiesterases / metabolism
  • Animals
  • Cyclic GMP / metabolism*
  • Cyclic GMP / pharmacology
  • Cyclic GMP-Dependent Protein Kinases / biosynthesis
  • Cyclic GMP-Dependent Protein Kinases / metabolism
  • Drosophila melanogaster / physiology*
  • Epithelium / drug effects
  • Epithelium / physiology
  • Gene Expression / drug effects
  • Homeostasis
  • Malpighian Tubules / drug effects
  • Malpighian Tubules / physiology*
  • NADPH Dehydrogenase / analysis
  • NADPH Dehydrogenase / metabolism
  • Nitric Oxide / pharmacology*
  • Nitroprusside / pharmacology*
  • Phosphoprotein Phosphatases / metabolism
  • RNA, Messenger / biosynthesis
  • Signal Transduction / drug effects
  • Signal Transduction / physiology*

Substances

  • RNA, Messenger
  • Nitroprusside
  • Nitric Oxide
  • NADPH Dehydrogenase
  • Cyclic GMP-Dependent Protein Kinases
  • Phosphoprotein Phosphatases
  • 3',5'-Cyclic-GMP Phosphodiesterases
  • Cyclic GMP