Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 653 (2), 117-22

Simplified Assay for the Quantification of 2,3-dinor-6-keto-prostaglandin F1 Alpha by Gas Chromatography-Mass Spectrometry

Affiliations

Simplified Assay for the Quantification of 2,3-dinor-6-keto-prostaglandin F1 Alpha by Gas Chromatography-Mass Spectrometry

V C Daniel et al. J Chromatogr B Biomed Appl.

Abstract

Endogenous prostacyclin production is best assessed by the measurement of its excreted metabolites, of which a major one is 2,3-dinor-6-ketoprostaglandin F1 alpha (2,3-dinor-6-keto-PGF1 alpha). Gas chromatographic-mass spectrometric (GC-MS) assays have been developed for this compound but are cumbersome and time-consuming. We now report a modified assay for the measurement of 2,3-dinor-6-keto-PGF1 alpha employing GC-MS in which sample preparation time is markedly shortened by replacing a number of extraction steps with reversed-phase column extraction and by modifying derivatization procedures. Precision of the assay is +/- 5% and the accuracy is 98%. The lower limit of detection in urine is approximately 15 pg/mg creatinine. Normal urinary levels of this metabolite were found to be 141 +/- 54 pg/mg creatinine (mean +/- S.D.). Urinary excretion of 2,3-dinor-6-keto-PGF1 alpha is markedly altered in situations associated with abnormalities of prostacyclin generation when quantified using this assay. Thus, this assay provides a sensitive and accurate method to assess endogenous prostacyclin production and to further explore the role of this compound in human health and disease.

Similar articles

See all similar articles

Cited by 20 PubMed Central articles

See all "Cited by" articles

Publication types

MeSH terms

Substances

Feedback