Biologically active recombinant human complement factor H: synthesis and secretion by the baculovirus system

Gene. 1994 Jun 10;143(2):301-2. doi: 10.1016/0378-1119(94)90116-3.

Abstract

A complete cDNA clone (4.3 kb) encoding human complement factor H (hCFH; 155 kDa) has been cloned into the pVL1393 baculovirus expression vector and transfected into Spodoptera frugiperda Sf9 insect cells. A biologically active (92 +/- 15%) 140-kDa protein was secreted into the medium with a yield of more than 5 mg/liter. This is the first report of synthesis of biologically active recombinant hCFH in a heterologous system.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Baculoviridae
  • Complement C3b / metabolism
  • Complement Factor H / biosynthesis*
  • Complement Factor H / metabolism
  • Genetic Vectors
  • Humans
  • Moths
  • Recombinant Proteins / biosynthesis*
  • Recombinant Proteins / metabolism

Substances

  • CFH protein, human
  • Recombinant Proteins
  • Complement C3b
  • Complement Factor H