Ogura cytoplasmic male-sterility (CMS)-associated orf138 is translated into a mitochondrial membrane polypeptide in male-sterile Brassica cybrids

Mol Gen Genet. 1994 Jun 3;243(5):540-7. doi: 10.1007/BF00284202.


Transcription of a putative mitochondrial gene (orf138) has previously been correlated with Ogura cytoplasmic male-sterility (CMS) in rapeseed cybrids. In this paper, studies performed on a Brassica cybrid with a different organization of the orf138 locus confirm this association. We also show that mitochondria isolated from male-sterile rapeseed plants synthesize a polypeptide of 19 kDa, which is absent in fertile revertants. Antibodies against a glutathione S-transferase-ORF138 fusion protein were raised to establish that this 19 kDa polypeptide is the product of orf138. The anti-ORF138 serum was used to demonstrate that the orf138 translation product occurs only in sterile cybrids and co-purifies with the mitochondrial membrane fraction.

MeSH terms

  • Blotting, Western
  • Brassica / genetics*
  • Brassica / physiology
  • Cloning, Molecular
  • DNA, Mitochondrial / biosynthesis*
  • Deoxyribonuclease BamHI
  • Deoxyribonucleases, Type II Site-Specific
  • Electrophoresis, Polyacrylamide Gel
  • Fertility / genetics
  • Hybridization, Genetic
  • Intracellular Membranes / chemistry
  • Membrane Proteins / biosynthesis*
  • Membrane Proteins / genetics
  • Molecular Sequence Data
  • Open Reading Frames
  • Plant Proteins / biosynthesis*
  • Plant Proteins / genetics
  • Plants / genetics*
  • Precipitin Tests
  • Protein Biosynthesis
  • Recombinant Fusion Proteins / biosynthesis
  • Restriction Mapping
  • Transcription, Genetic


  • DNA, Mitochondrial
  • Membrane Proteins
  • Plant Proteins
  • Recombinant Fusion Proteins
  • Deoxyribonuclease BamHI
  • endodeoxyribonuclease NcoI
  • Deoxyribonucleases, Type II Site-Specific