Cloning of novel Wilms tumor gene (WT1) cDNAs; evidence for antisense transcription of WT1

Oncogene. 1994 Jul;9(7):2059-63.

Abstract

WT1 is a tumor suppressor gene that has been implicated in Wilms tumor, and is expressed in cells of mesodermal origin. The Wit-1 gene is located approximately 2 kb from the WT1 gene, and is expressed coordinately with WT1. WT1 and Wit-1 are bi-directionally transcribed from the same promoter region. We have screened a human fetal kidney cDNA library to identify novel WT1 cDNA clones. Here we report the cloning of cDNA clones which span part of intron 1 of WT1, exon 1, upstream sequences between WT1 and Wit-1 and part of the Wit-1 gene. Northern blot and RNAase protection analysis using subcloned fragments of the cDNAs corresponding to regions from within intron 1 of WT1 suggest that a 7-10 Kb RNA is expressed in human fetal kidney, which overlaps with WT1 and is transcribed in the same direction as Wit-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • Genes, Wilms Tumor*
  • Humans
  • Introns
  • Kidney / embryology
  • Kidney / metabolism
  • Molecular Sequence Data
  • Oligonucleotides, Antisense / genetics*
  • Transcription, Genetic*

Substances

  • DNA, Complementary
  • Oligonucleotides, Antisense