JHM virus-specific cytotoxic T cells derived from the central nervous system

Adv Exp Med Biol. 1993;342:419-23. doi: 10.1007/978-1-4615-2996-5_66.

Abstract

Spleen cells cultured from Balb/c mice immunized with the JHM strain of mouse hepatitis virus (JHMV) have CD8+ cytotoxic T cells (CTL) specific for both the S and N proteins, but not the M or HE proteins. T cell lines were established from the brains of Balb/c mice infected with JHMV. The majority of the lines (20 of 22) were specific for JHMV. Analysis of the viral structural proteins which served as target structures indicate that most (15 of 20) were specific for the N protein. One line was specific for the S protein and four lines were specific for JHMV but the protein recognized could not be determined. These data suggest that early during infection there is a preferential recruitment of N protein specific CTL into the CNS of infected mice.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Brain / immunology
  • Brain / pathology*
  • Capsid / immunology*
  • Cell Line
  • Coronavirus Infections / immunology*
  • Coronavirus Infections / pathology
  • Encephalitis / immunology*
  • Encephalitis / pathology
  • Membrane Glycoproteins / immunology
  • Mice
  • Mice, Inbred BALB C
  • Murine hepatitis virus / immunology*
  • Spike Glycoprotein, Coronavirus
  • T-Lymphocytes, Cytotoxic / immunology*
  • Vaccination
  • Vaccines, Synthetic / immunology
  • Vaccinia virus
  • Viral Core Proteins / immunology*
  • Viral Envelope Proteins / immunology
  • Viral Vaccines / immunology

Substances

  • Membrane Glycoproteins
  • Spike Glycoprotein, Coronavirus
  • Vaccines, Synthetic
  • Viral Core Proteins
  • Viral Envelope Proteins
  • Viral Vaccines
  • spike glycoprotein, SARS-CoV
  • spike protein, mouse hepatitis virus