Interaction of mammalian splicing factor SF3a with U2 snRNP and relation of its 60-kD subunit to yeast PRP9

Science. 1993 Oct 1;262(5130):102-5. doi: 10.1126/science.8211112.

Abstract

In the assembly of a prespliceosome, U2 small nuclear ribonucleoprotein (snRNP) functions in pre-messenger RNA (mRNA) splicing together with splicing factors (SFs) 3a, SF3b, and several other proteins. The 17S but not the 12S form of U2 snRNP is active in splicing-complex formation. Here it is shown that the SF3a subunits correspond to three of the 17S U2 snRNP-specific polypeptides. SF3a interacts with U2 snRNP in the presence of SF3b to generate a structure similar to 17S U2 snRNP, which suggests a function for SF3a and SF3b in the incorporation of U2 snRNP into the spliceosome. Furthermore, the 60-kilodalton subunit of SF3a is related to the yeast splicing protein PRP9.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Cross Reactions
  • Electrophoresis, Polyacrylamide Gel
  • Fungal Proteins / chemistry
  • Fungal Proteins / immunology
  • Fungal Proteins / metabolism*
  • Humans
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / immunology
  • Nuclear Proteins / metabolism*
  • RNA Splicing Factors
  • RNA Splicing*
  • RNA-Binding Proteins
  • Ribonucleoprotein, U2 Small Nuclear / chemistry
  • Ribonucleoprotein, U2 Small Nuclear / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Spliceosomes / metabolism*

Substances

  • Fungal Proteins
  • Nuclear Proteins
  • PRP9 protein, S cerevisiae
  • RNA Splicing Factors
  • RNA-Binding Proteins
  • Ribonucleoprotein, U2 Small Nuclear
  • Saccharomyces cerevisiae Proteins
  • Adenosine Triphosphate