Correspondence between a mammalian spliceosome component and an essential yeast splicing factor

Science. 1993 Oct 1;262(5130):105-8. doi: 10.1126/science.8211113.

Abstract

None of the mammalian splicing factors that have been cloned corresponds to the yeast pre-messenger RNA splicing factors, the PRP proteins. Here, a generalizable strategy was used to isolate a complementary DNA encoding the mammalian spliceosome-associated protein (SAP) SAP 62. It is demonstrated that SAP 62 is the likely functional homolog of the yeast PRP11 protein. Both PRP11 and SAP 62 associate stably with the spliceosome, contain a single zinc finger, and display significant amino acid sequence similarity. Unlike PRP11, SAP 62 contains 22 proline-rich heptapeptide repeats at the carboxyl-terminus.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Carrier Proteins / chemistry*
  • Carrier Proteins / metabolism
  • Fungal Proteins / chemistry*
  • Fungal Proteins / metabolism
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Nucleoproteins / chemistry*
  • Nucleoproteins / metabolism
  • RNA Precursors / metabolism
  • RNA Splicing Factors
  • RNA Splicing*
  • RNA-Binding Proteins*
  • Repetitive Sequences, Nucleic Acid
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Spliceosomes / chemistry*
  • Spliceosomes / metabolism
  • Zinc Fingers

Substances

  • Carrier Proteins
  • Fungal Proteins
  • Nucleoproteins
  • PRP11 protein, S cerevisiae
  • RNA Precursors
  • RNA Splicing Factors
  • RNA-Binding Proteins
  • SF3A2 protein, human
  • Saccharomyces cerevisiae Proteins

Associated data

  • GENBANK/L21990