Cloning and DNA Sequence of a 29 Kilodalton Polypeptide Gene of Mycoplasma Gallisepticum as a Possible Protective Antigen

Vaccine. 1993;11(10):1061-6. doi: 10.1016/0264-410x(93)90134-j.

Abstract

A lambda gt11 clone, designated M1 and having a 0.8 kilobase (kb) insert, was selected by screening a Mycoplasma gallisepticum (M.g.) genomic DNA library with antisera against M.g. cells and their membrane proteins. The sequence of a 1.7 kb EcoRI fragment of genomic DNA covering the entire M1 insert revealed a long open reading frame, TM-1, that encoded a polypeptide with a deduced molecular weight of 29 kDa. An antiserum raised in chicken against the TM-1 polypeptide, which was produced by recombinant Escherichia coli cells and purified by column chromatography, inhibited growth of M.g. cells in vitro. Moreover, chickens immunized with this polypeptide were partially protected from a challenge with virulent M.g. This polypeptide may serve as the basis for a vaccine against M.g. infection.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Bacterial / genetics*
  • Antigens, Bacterial / immunology
  • Antigens, Bacterial / isolation & purification
  • Base Sequence
  • Chickens
  • Cloning, Molecular*
  • Codon
  • DNA, Bacterial / chemistry*
  • Escherichia coli / metabolism
  • Genes, Bacterial*
  • Immune Sera / immunology
  • Immunization
  • Molecular Sequence Data
  • Molecular Weight
  • Mycoplasma / genetics
  • Mycoplasma / immunology*

Substances

  • Antigens, Bacterial
  • Codon
  • DNA, Bacterial
  • Immune Sera