Determination of the 13C-labeling pattern of glucose by gas chromatography-mass spectrometry

Anal Biochem. 1993 Aug 1;212(2):526-31. doi: 10.1006/abio.1993.1363.

Abstract

We developed a gas chromatography-mass spectrometric method which allows to determine the complete 13C-labeling pattern of glucose. The method uses four derivatives of glucose (methyloxime trimethylsilyl, bisbutylboronate acetate, aldonitrile pentaacetate, and permethyl) and selective analysis of fragment ions retaining specific carbon atoms. The technique was tested by analyzing glucose from rat livers perfused with various 13C tracers. The labeling patterns agree with theoretical calculations and with literature reports where [14C]glucose was analyzed by degradation and [13C]glucose was analyzed by NMR.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Carbon Isotopes
  • Gas Chromatography-Mass Spectrometry / methods
  • Glucose / analysis*
  • Glucose / chemistry
  • Glucose / metabolism
  • In Vitro Techniques
  • Isotope Labeling / methods*
  • Liver / metabolism
  • Mass Spectrometry
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Carbon Isotopes
  • Glucose