Inhibition of the Infectivity of Influenza Virus by Tea Polyphenols

Antiviral Res. 1993 Aug;21(4):289-99. doi: 10.1016/0166-3542(93)90008-7.

Abstract

(-)Epigallocatechin gallate (EGCg) and theaflavin digallate (TF3) (1-10 microM) inhibited the infectivity of both influenza A virus and influenza B virus in Madin-Darby canine kidney (MDCK) cells in vitro. Study by electron microscope revealed that EGCg and TF3 (1 mM) agglutinated influenza viruses as well as did antibody, and that they prevented the viruses from adsorbing to MDCK cells. EGCg and TF3 more weakly inhibited adsorption of the viruses to MDCK cells. EGCg and TF3 (1-16 microM) also inhibited haemagglutination by influenza viruses. These findings suggest that tea polyphenols bind to the haemagglutinin of influenza virus, inhibit its adsorption to MDCK cells, and thus block its infectivity.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antiviral Agents / metabolism
  • Antiviral Agents / pharmacology*
  • Biflavonoids*
  • Catechin / analogs & derivatives*
  • Catechin / metabolism
  • Catechin / pharmacology
  • Cell Line
  • Dogs
  • Gallic Acid / analogs & derivatives*
  • Gallic Acid / metabolism
  • Gallic Acid / pharmacology
  • Hemagglutination, Viral / drug effects
  • Influenza A virus / drug effects*
  • Influenza A virus / growth & development
  • Influenza A virus / pathogenicity*
  • Influenza B virus / drug effects*
  • Influenza B virus / growth & development
  • Influenza B virus / pathogenicity*
  • Kidney / drug effects
  • Kidney / microbiology
  • Microscopy, Electron, Scanning
  • Orthomyxoviridae Infections / drug therapy
  • Orthomyxoviridae Infections / microbiology
  • Plant Extracts / pharmacology*
  • Rabbits
  • Tea
  • Viral Plaque Assay

Substances

  • Antiviral Agents
  • Biflavonoids
  • Plant Extracts
  • Tea
  • theaflavin digallate
  • Gallic Acid
  • Catechin
  • epigallocatechin gallate