The effect of reactive oxygen species on the biosynthesis of collagen and glycosaminoglycans in cultured human dermal fibroblasts

Arch Dermatol Res. 1993;285(6):352-5. doi: 10.1007/BF00371836.


The purpose of this study was to evaluate the possibility that the biological changes observed in connective tissue matrix components of photoaging skin may be induced by an alteration of biosynthesis in fibroblasts damaged by reactive oxygen species (ROS). We investigated the effect of ROS induced by xanthine and the xanthine oxidase system on the biosynthesis of connective tissue matrix components, collagen and glucosaminoglycans (GAGs) in cultured human dermal fibroblasts. ROS decreased collagen production and increased GAGs synthesis. Interestingly, these changes were consistent with the biological alterations of connective tissue matrix components observed in photoaging skin. Moreover, catalase and alpha-tocopherol completely prevented the ROS-induced alterations of collagen and GAGs biosynthesis, whereas superoxide dismutase had no effect on the ROS-induced changes. These results suggest that ROS may be one of the factors which cause the biological changes of connective tissue matrix components observed in photoaging skin.

MeSH terms

  • Catalase / pharmacology
  • Cells, Cultured
  • Collagen / biosynthesis*
  • Connective Tissue / drug effects
  • Connective Tissue / metabolism
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Free Radical Scavengers
  • Glycosaminoglycans / biosynthesis*
  • Humans
  • Reactive Oxygen Species / adverse effects*
  • Reactive Oxygen Species / metabolism
  • Skin / drug effects*
  • Skin / metabolism
  • Skin Aging / drug effects
  • Skin Aging / physiology
  • Skin Aging / radiation effects
  • Ultraviolet Rays / adverse effects
  • Vitamin E / pharmacology
  • Xanthine
  • Xanthine Oxidase / pharmacology
  • Xanthines / pharmacology


  • Free Radical Scavengers
  • Glycosaminoglycans
  • Reactive Oxygen Species
  • Xanthines
  • Vitamin E
  • Xanthine
  • Collagen
  • Catalase
  • Xanthine Oxidase