Molecular cloning of cDNA encoding human drebrin E and chromosomal mapping of its gene

Biochem Biophys Res Commun. 1993 Oct 15;196(1):468-72. doi: 10.1006/bbrc.1993.2273.

Abstract

Drebrins are novel actin-binding proteins in the brain which are developmentally regulated. Three isoforms: two embryonic types (E1 and E2) and an adult type (A) are generated by alternative RNA splicing from a single debrin gene in the chicken brain. A full length cDNA clone of human drebrin E has been isolated from a cDNA library of human fetus brain. The clone is 2596 base pairs in length and contains an open reading frame of 1947 nucleotides encoding a protein of 649 amino acids. The deduced amino acid sequence, except for the internal 138-nucleotide sequence (ins2), exhibits 88% homology with rat drebrin A. Spot blot hybridization using flow-sorted human chromosomes provides evidence that the gene encoding human drebrin protein locates on human chromosome 5.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Brain / physiology
  • Chromosome Mapping*
  • Chromosomes, Human, Pair 5*
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Female
  • Fetus
  • Flow Cytometry
  • Humans
  • Karyotyping
  • Molecular Sequence Data
  • Neuropeptides / genetics*
  • Nucleic Acid Hybridization
  • Pregnancy
  • Pregnancy Trimester, Second
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • Neuropeptides
  • drebrin E
  • drebrins

Associated data

  • GENBANK/D17530
  • GENBANK/L14791
  • GENBANK/L14792
  • GENBANK/L14793
  • GENBANK/L14794
  • GENBANK/L14795
  • GENBANK/L14796
  • GENBANK/L14797
  • GENBANK/L14798
  • GENBANK/U03866