Large granular lymphocytosis in a patient infected with HTLV-II

AIDS Res Hum Retroviruses. 1993 Aug;9(8):715-9. doi: 10.1089/aid.1993.9.715.


HTLV-II has been associated with a variety of lymphoproliferative disorders, including atypical hairy cell leukemia, chronic T cell leukemia, T prolymphocytic leukemia, and large granular lymphocytic leukemia. However, a direct or indirect role for HTLV-II in these disorders is not yet firmly established. We studied a patient diagnosed as having leukemia of the large granular lymphocyte (LGL) type who was HTLV-II seropositive, to determine if the expanded cell population was infected. Two populations of CD3-CD16+ LGL were identified; one was CD8+, the other CD8-. Populations of cells with these surface markers as well as normal CD3+CD4+ and CD3+CD8+ cells were separated by flow cytometric methods, DNA extracted, and gene regions of HTLV-II pol and tax amplified, using the polymerase chain reaction, and probed after Southern blotting. HTLV-II was detected in the CD3+CD8+ population, and not in the CD3-CD16+ large granular lymphocyte population. This finding indicates that the role of HTLV-II, if any, in LGL proliferation is indirect.

Publication types

  • Case Reports
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, CD / analysis
  • DNA, Viral / analysis
  • Flow Cytometry
  • Genes, pX
  • Genes, pol
  • HTLV-II Infections / complications*
  • Human T-lymphotropic virus 2 / genetics
  • Humans
  • Immunophenotyping
  • Leukemia, Lymphoid / complications*
  • Lymphocytosis / complications*
  • Male
  • Middle Aged


  • Antigens, CD
  • DNA, Viral