A rapid, automated microtiter assay for measuring neutralization of HIV-1

AIDS Res Hum Retroviruses. 1993 Aug;9(8):781-5. doi: 10.1089/aid.1993.9.781.


A sensitive, rapid, and quantitative ELISA for p24 is described, which can be used as the read-out to test for HIV-1 neutralization in the syncytium-forming microassay and can replace the counting of syncytia under the microscope. This assay can be used reliably for divergent strains of HIV-1, including those that do not induce syncytia. The new read-out permits evaluation of neutralization at 3 days rather than 5 days. Using this assay, the neutralizing activity of several new and previously described human monoclonal antibodies against HIV-1 was characterized.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Giant Cells / pathology
  • HIV Core Protein p24 / analysis
  • HIV-1 / immunology*
  • Humans
  • Kinetics
  • Neutralization Tests*


  • HIV Core Protein p24