Identification and localization of ERD2 in the malaria parasite Plasmodium falciparum: separation from sites of sphingomyelin synthesis and implications for organization of the Golgi

EMBO J. 1993 Dec;12(12):4763-73.


The ERD2 gene product in mammalian cells and yeast is a receptor required for protein retention in the endoplasmic reticulum (ER); immunolocalization studies indicate that the protein is concentrated in the cis Golgi. We have identified a homologue of ERD2 in the malaria parasite, Plasmodium falciparum (PfERD2). The deduced protein sequence is 42% identical to mammalian and yeast homologues and bears striking homology in its proposed tertiary structure. PfERD2 is tightly confined to a single focus of staining in the perinuclear region as seen by indirect immunofluorescence. This is redistributed by brefeldin A (BFA) to a diffuse pattern similar to that of parasite BiP, a marker for the ER; removal of the drug results in recovery of the single focus, consistent with the localization of PfERD2 to the parasite Golgi and its participation in a retrograde transport pathway to the ER. Sphingomyelin synthesis is a second resident activity of the cis Golgi whose organization is sensitive to BFA in mammalian cells. Within the parasite it again localizes to a perinuclear region but does not reorganize upon BFA treatment. The results strongly suggest that these two activities are in distinct compartments of the Golgi in the malaria parasite.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Protozoan / immunology
  • Base Sequence
  • Brefeldin A
  • Cloning, Molecular
  • Cyclopentanes / pharmacology
  • Fluorescent Antibody Technique
  • Golgi Apparatus / metabolism*
  • Membrane Proteins / immunology
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Plasmodium falciparum / metabolism*
  • Protozoan Proteins*
  • Receptors, Peptide*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Sphingomyelins / biosynthesis*


  • Antibodies, Protozoan
  • Cyclopentanes
  • ERD2 protein, Plasmodium
  • ERD2 protein, S cerevisiae
  • Membrane Proteins
  • Oligodeoxyribonucleotides
  • Protozoan Proteins
  • Receptors, Peptide
  • Saccharomyces cerevisiae Proteins
  • Sphingomyelins
  • Brefeldin A

Associated data

  • GENBANK/X74869