Subtraction radiography offers greater visualization of radiographic changes between a pair of radiographs by subtracting out the unchanging background distractions. Originally, subtraction was achieved by using positive and negative prints in angiography. The advent of digital imaging devices has led to validation of quantitative measurements from digital subtraction images. As well, computer software manipulations have allowed for more flexibility in the clinical application of subtraction imaging. The clinical application of digital subtraction radiography is discussed along with clinical cases.