Epoc-1: a POU-domain gene expressed in murine epidermal basal cells and thymic stromal cells

Gene. 1993 Nov 15;133(2):163-9. doi: 10.1016/0378-1119(93)90634-f.


POU-domain transcription factors are known as developmental regulators which control organ development and cell phenotypes. In order to clarify the roles of POU-domain transcription factors in cell differentiation, we cloned a novel POU family gene, Epoc-1, from a murine thymus cDNA library. The amino acid (aa) sequence of the POU-specific domain of Epoc-1 is almost identical to those of Oct-1 and Oct-2. However, within the POU-homeodomain, 13 out of 60 aa differ between Epoc-1 and Oct-2. Recombinant Epoc-1 products were found to bind specifically to the octamer sequence. Epoc-1 was found to be expressed in skin, thymus, stomach and testis. In situ hybridization experiments and RNase protection assays indicated that Epoc-1 is expressed in the epidermal basal cells of the skin, which contain stem cells unipotent for keratinocyte differentiation and in thymic stromal elements. These results suggest that Epoc-1 might be one of the developmental regulators which controls epidermal development and thymic organogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Differentiation / genetics
  • Cloning, Molecular
  • DNA
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Epidermal Cells
  • Epidermis / metabolism
  • Gene Expression
  • Host Cell Factor C1
  • Mice
  • Molecular Sequence Data
  • Multigene Family
  • Octamer Transcription Factor-1
  • Octamer Transcription Factor-2
  • Octamer Transcription Factors
  • Organ Specificity
  • POU Domain Factors
  • Sequence Homology, Amino Acid
  • Stromal Cells / metabolism
  • Thymus Gland / cytology
  • Thymus Gland / metabolism*
  • Transcription Factors / biosynthesis
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism


  • DNA-Binding Proteins
  • Hcfc1 protein, mouse
  • Host Cell Factor C1
  • Octamer Transcription Factor-1
  • Octamer Transcription Factor-2
  • Octamer Transcription Factors
  • POU Domain Factors
  • POU2F3 protein, human
  • Pou2f1 protein, mouse
  • Pou2f2 protein, mouse
  • Pou2f3 protein, mouse
  • Transcription Factors
  • DNA

Associated data

  • GENBANK/D38370
  • GENBANK/D38371
  • GENBANK/L08645
  • GENBANK/L14677
  • GENBANK/L24524
  • GENBANK/L24525
  • GENBANK/L24526
  • GENBANK/L24527
  • GENBANK/L24528
  • GENBANK/L24529