Spectral sensitivity of the blood-retinal barrier at the pigment epithelium for blue light in the 400-500 nm range

Graefes Arch Clin Exp Ophthalmol. 1993 Oct;231(10):600-6. doi: 10.1007/BF00936526.


To specify the spectral sensitivity of the retinal pigment epithelium (RPE) for blue light damage, pigmented rabbits were exposed to light of 408, 418, 439, 455, 485, and 500 nm (half-peak bandwidth approximately 12 nm). The range of radiant exposure was 15-275 J cm-2 (1.7-19 mW cm-2 for 0.5-5 h). Vitreous fluorophotometry was used to functionally evaluate the blood-retinal barrier at the RPE in vivo, and electron microscopy to visualize RPE ultrastructure in vitro. A significant increase in permeability of the blood-retinal barrier was seen only after exposure to light of 418 nm. Radiant exposure at threshold for permeability increase was 18 J cm-2. Electron microscopy of the RPE demonstrated dispersion and clumping of melanin granules. The results suggest that the RPE is most sensitive to light in the range 412-425 nm, possibly due to damage-mediating chromophores such as cytochrome c oxidase and lipofuscin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood-Retinal Barrier / radiation effects*
  • Cell Membrane Permeability / radiation effects
  • Fluorophotometry
  • Fundus Oculi
  • Light / adverse effects*
  • Pigment Epithelium of Eye / radiation effects*
  • Pigment Epithelium of Eye / ultrastructure
  • Rabbits
  • Radiation Injuries, Experimental / pathology
  • Radiation Injuries, Experimental / physiopathology*
  • Sensitivity and Specificity
  • Sensory Thresholds
  • Vitreous Body / physiology