Overexpression of YAP2, coding for a new yAP protein, and YAP1 in Saccharomyces cerevisiae alleviates growth inhibition caused by 1,10-phenanthroline

J Biol Chem. 1993 Nov 5;268(31):23640-5.


A new member of the yeast yAP family, designated YAP2, has been isolated and characterized. The protein displays a high homology with the DNA-binding domain of yAP-1, which contains a basic DNA-binding domain and leucine zipper motif and binds in vitro to the same cis-element. Growth arrest of yeast caused by low concentrations of 1,10-phenanthroline, resulting in zinc and/or iron deprivation, is overcome by over-expression of YAP1 or YAP2. In fact, yeast cells over-expressing YAP1 or YAP2 display pleiotropic drug resistance. On the other hand, a yap2 null mutant has an increased thermotolerance under starvation conditions caused by 1,10-phenanthroline. The latter mutant might become an excellent tool in the study of pathways leading toward thermotolerance acquisition.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Division / drug effects*
  • Cloning, Molecular
  • DNA-Binding Proteins / physiology*
  • Fungal Proteins / physiology
  • Hot Temperature*
  • Molecular Sequence Data
  • Multigene Family
  • Mutagenesis, Insertional
  • Phenanthrolines / pharmacology*
  • Restriction Mapping
  • Saccharomyces cerevisiae / drug effects*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Sequence Deletion
  • Sequence Homology, Amino Acid
  • Transcription Factors / physiology*


  • CAD1 protein, S cerevisiae
  • DNA-Binding Proteins
  • Fungal Proteins
  • Phenanthrolines
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • YAP1 protein, S cerevisiae
  • 1,10-phenanthroline

Associated data

  • GENBANK/X69106