Cloning and sequencing of two new Verotoxin 2 variant genes of Escherichia coli isolated from cases of human and bovine diarrhea

Microbiol Immunol. 1993;37(6):451-9. doi: 10.1111/j.1348-0421.1993.tb03236.x.

Abstract

We cloned and sequenced two new Verotoxin 2 (VT2) variant genes: one from an Escherichia coli strain from a case of bovine diarrhea and the other from an E. coli strain from a patient with diarrhea. The nucleotide and amino acid sequences of these two genes were highly homologous with, but distinct from those of the VT2, VT2vha, VT2vhb, SLT-IIv (VT2vp1) and SLT-IIva (VT2vp2) genes. Their nucleotide sequences were much more closely homologous to that of VT2vh than to that of VT2vp. Search for these two new genes in other Verocytotoxin-producing E. coli strains resulted in the isolation of 2 strains carrying one of the new VT2 variant genes, one strain from Tokyo and the other from Canada.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Toxins / genetics*
  • Base Sequence
  • Cattle
  • Cattle Diseases / microbiology
  • Cloning, Molecular
  • DNA Probes
  • DNA, Bacterial / genetics
  • Diarrhea / microbiology*
  • Diarrhea / veterinary
  • Enterotoxins / genetics
  • Escherichia coli / genetics*
  • Escherichia coli Infections / microbiology*
  • Genetic Variation*
  • Humans
  • Molecular Sequence Data
  • Restriction Mapping
  • Sequence Homology
  • Shiga Toxin 2
  • Vero Cells

Substances

  • Bacterial Toxins
  • DNA Probes
  • DNA, Bacterial
  • Enterotoxins
  • Shiga Toxin 2