Effects of certain inflammatory mediators on bovine neutrophil migration in vivo and in vitro

Vet Immunol Immunopathol. 1993 Jul;37(2):99-112. doi: 10.1016/0165-2427(93)90058-c.

Abstract

Migration of bovine neutrophils towards endotoxin, recombinant bovine interleukin-1 beta (rBoIL-1 beta), recombinant human tumor necrosis factor-alpha (rhTNF-alpha), platelet-activating factor (PAF), complement factor C5a, leukotriene B4 (LTB4), and recombinant human interleukin-8 (rhIL-8) was studied in vivo, using the teat cistern model, and in vitro using the modified Boyden chamber method. Infusion of endotoxin, rBoIL-1 beta, rhTNF-alpha, PAF, or C5a into the teat cistern induced significant accumulation of leukocytes, mainly neutrophils, during the sampling period. Endotoxin was, on a molar basis, the most potent inducer of cell accumulation in vivo, followed by rBoIL-1 beta, while C5a, PAF and rhTNF-alpha were less potent. No significant cell accumulation was observed after infusion of LTB4 or rhIL-8. A significant migration of cells into the teat cistern was first observed 2 h after the infusion of endotoxin or rBoIL-1 beta, the rBoIL-1 beta-induced response started somewhat earlier. The first significant cell accumulation after infusion of PAF or C5a was observed already 1.5 h post infusion. The largest numbers of cells were reached 2.5-4.5 h after the infusion of endotoxin, rBoIL-1 beta, rhTNF-alpha, PAF or C5a. In vitro, significant migration of bovine blood neutrophils was observed towards C5a or rhIL-8, and to a lower extent towards LTB4, while no chemotactic response to endotoxin, rBoIL-1 beta, rhTNF-alpha, and PAF was observed. Possible roles of the different substances as inducers of neutrophil migration into the bovine teat are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Toxins / immunology
  • Cattle
  • Cell Movement
  • Chemotaxis, Leukocyte
  • Cytokines / immunology*
  • Endotoxins / immunology
  • Female
  • Leukocyte Count
  • Leukotriene B4 / immunology
  • Mammary Glands, Animal / immunology*
  • Mastitis, Bovine / immunology*
  • Neutrophils / immunology*
  • Recombinant Proteins / immunology

Substances

  • Bacterial Toxins
  • Cytokines
  • Endotoxins
  • Recombinant Proteins
  • salmonella toxin
  • Leukotriene B4