The influence of IL-3, IL-5, and GM-CSF on normal human eosinophil and neutrophil C3b-induced degranulation

Allergy. 1993 Aug;48(6):437-42.

Abstract

The priming effect of interleukin-3 (IL-3), interleukin-5 (IL-5), and granulocyte/macrophage-colony stimulating factor (GM-CSF) on eosinophil and neutrophil degranulation was studied. Granulocytes were obtained from normal donors, and degranulation was induced by incubation with serum-opsonized Sephadex particles. The released amounts of eosinophil cationic protein (ECP), eosinophil protein X (EPX), myeloperoxidase (MPO), and lactoferrin (LF) were measured by radioimmunoassay (RIA). The effect of IL-5 was dose- and time-dependent, with a maximal enhancement of ECP and EPX release of 71% (P < 0.03) and 66% (P < 0.03), respectively. Neutrophil degranulation, however, was unaffected. IL-3 was marginally effective, whereas GM-CSF seemed to act as a secretagogue for both eosinophil and neutrophil degranulation. We conclude that IL-5 selectively primes eosinophil degranulation, whereas IL-3 and GM-CSF seem to act as secretagogues for eosinophils and neutrophils. The results indicate that IL-5 may be involved in the priming of eosinophils as observed in patients with asthma and hypereosinophilic syndrome (HES).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Proteins / drug effects
  • Blood Proteins / metabolism
  • Cell Degranulation / drug effects*
  • Complement C3b / pharmacology
  • Eosinophil Granule Proteins
  • Eosinophil-Derived Neurotoxin
  • Eosinophils / drug effects
  • Eosinophils / physiology*
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology*
  • Humans
  • Interleukin-3 / pharmacology*
  • Interleukin-5 / pharmacology*
  • Neutrophils / drug effects
  • Neutrophils / physiology*
  • Ribonucleases*

Substances

  • Blood Proteins
  • Eosinophil Granule Proteins
  • Interleukin-3
  • Interleukin-5
  • Complement C3b
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Eosinophil-Derived Neurotoxin
  • Ribonucleases